Pre-Ph.D. Course Work

Duration of Pre-Ph.D. Course: 2 semesters
Pre-Ph.D. Course:
FIRST SEMESTER (12 Credits)
Molecular diagnostics (3 Credits)
Cell Signalling and Cancer (3 Credits)
Cancer & cell cycle (3 Credits)
Metabolic Disorders (3 Credits)
Molecular basis of Diseases (3 Credits)
Concepts and perspective of molecular medicine (3 Credits)
Molecular Endocrinology / Nuclear Receptors in Health and Disease (3 Credits)
Gene Expression and Regulation (3 Credits)
Molecular Microbiology / Molecular basis of infectious diseases (3 Credits)
Epigenetics (3 Credits)
Bioinformatics and Biostatistics (3 Credits)
Research Methodology (3 Credits)
SECOND SEMESTER (6 Credits)
Presentation of a research proposal (3 Credit)

Laboratory Techniques (3 Credit)

• Immunocytochemistry
• ELISA
• Electrophoretic and hybridization techniques.
• Western, Northern and Southern blotting.
• Ultracentrifugation, liquid chromatographic techniques.
• Polymerase Chain Reaction (PCR), Reverse Transcriptase PCR and Real Time PCR.
• Electrophoretic mobility shift assay (EMSA), DNA sequencing, DNA footprinting.
• Bacterial, fungal and mammalian cell culture.
• Transient and stable DNA transfection.
• Karyotyping.
• Fluorescence and confocal microscopy.
• Reporter gene assays.
• Antibody Generation (Polyclonal and Monoclonl)
• Use and analysis of radioisotopes.
• Antibody Generation (Polyclonal and Monoclonl)
• Genomics, microarray and proteomics – theory and applications.
• DNA (Plasmid/ genomic DNA) and RNA isolation.