Dr. Seema Negi

Research Officer,
Central Research Station, Subharti Medical College,
Swami Vivekanand Subharti University, Meerut-250005, India
Phone No.: (+91-121) 2439056/3058034, Ext. 2160, 2144
Email: seemanegi03@yahoo.co.in
Web: seemanegi.fusionarrow.com

Nuclear Receptors in Health and Disease

Regulation of Transcription and Signalling Pathways involved in Cancer and Metabolism

Screening for New Chemical Entity (NCE)

New Drug and Target Discovery Research

• Ph. D. (Molecular Medicine: Molecular Cellular Biochemistry & Molecular Pharmacology)
  Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India

• M. E. (Biotechnology)
  Birla Institute of Technology and Science (BITS) , Pilani, Rajasthan , India

• B. Pharm. (Dept. of Pharm. Sciences)
  HNB Garhwal University, Srinagar, Uttarakhand, India

• D. Pharm.
  Govt. Polytechnic, Narendra Nagar, Tehri, Uttarakhand, India

Ph.D. Thesis Title: Modulation of Pregnane & Xenobiotic Receptor functions by prospective anti-cancer herbal drugs.

Other Professional Qualification :

Completed the ‘General course in Intellectual Property’ via Distance Learning Programme organized by World Intellectual Property Organization (WIPO), Geneva, Germany.

Duly Registered as Registered Pharmacist in Uttaranchal Pharmacy Council, Dehradun on successful completion of D. Pharma and B. Pharma.

Currently working as Research Officer Central Research Station, Subharti Medical College SVSU Meerut from July 2014.

Worked as Senior Research Fellow at Peptide Biology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi under Prof. Kasturi Mukhopadhyay in ICMR funded project entitled ‘Structure-activity relationship of alpha-Melancocyte Stimulating Hormone: Mechanism of Staphylocidal activity’ from 26^th March, 2014 to 30th June 2014.

Worked as Ph.D. Scholar at Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi from 2008 to 2013 under Prof. R. K. Tyagi.

Worked as Senior Research Fellow in CSIR funded project entitled ‘ Interrogating the role of a novel xenosensor ‘Steriod and Xenobiotic receptor’ in metabolism and elimination of endocrine disruptors and pesticides ’ under Prof. R. K. Tyagi from 20^th Sep. 2006 to 19^th Sep. 2008 at Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi.

Six months M. E. Biotechnology Project entitled ‘ Status of Nitric oxide in Striatum of Maneb and Paraquat Induced Parkinson's Disease Phenotype in Mouse: A Molecular Approach’ at Environmental Biotechnology Div., Industrial Toxicology Research Centre (ITRC, CSIR) Lucknow.

Worked as summer trainee for ten week project entitled ‘ Differential Expression and Induction of yejF gene in Salmonella typhimurium’ during May-July 2005 at Dept. of Microbiology and Cell Biology, Indian Institute of Science (IISc), Bangalore, India.

Worked as summer trainee for ten week project entitled‘Antimicrobial Screening & Identification of Actinomycetes’, at Regional Research Laboratory (RRL-CSIR), Jammu (J&K), India.

Worked during M.E. Biotechnology on short project entitled ‘Cloning and Analysis of STARP gene from Plasmodium falciparum’ at BITS, Pilani.

Recipient of Best Oral Presentation Award in National Conference on Recent Advances in Biosciences and Drug Discovery (RABDD-2014) during 03-04th Mar., 2014 organized by Gurukul Kangri University, Haridwar, Uttarakhand, India.

Recipient of N. J. Chinoy Best Poster Award in ‘National Conference & 30th Annual Symposium of the Society for Reproductive Biology and Comparative Endocrinology (SRBCE) on Novel Aspects and Emerging Trends in Reproduction and Endocrinology’ during 30^th Jan to 1^st Feb, 2012.

Awarded Direct Senior Research Fellowship from Council of scientific and Industrial Research (CSIR), India (2009-2013).

Qualified GATE-2004 and secured 85.66 percentile organized by IIT Delhi.

Third topper in B. Pharm. in the HNB Garhwal University, Uttarakhand.

Awarded scholarship from ‘Mahabir Prasad Singh Foundation for Education and Research, New Delhi’ on All India Competitive Basis.

Awarded ‘Dr. Pushpashekhranand Dhyani Merit Scholarship’ for 2003-04 by Dr. Pushpashekhranand Dhyani Trust, Dehradun.

Best Scholar Award for being Topper in B. Pharm. I^St Year.

Best Girl Scholar Award for being topper in Girls in D. Pharm.

Highly Appreciated Scientific Model Award in Science Exhibition for the model on ‘Locomotion in Hydra’ Organized by Vidya Mandir Inter College, Haridwar.

Life Member of ‘Society for Reproductive Biology and Comparative Endocrinology (SRBCE)’ Member Id No. LM270-2009.

Tyagi R. K., Negi S., Kumari S., Saradhi M. and Mukhopadhyay G. Stable integration of a xenobiotic receptor promoter-reporter DNA construct into a human liver cell line for evaluating the drugs by high throughput screening. Indian patent application number: 464/DEL/2010.

International (PCT) patent application number: PCT/IN2011/000127.

Publication number: WO/2011/108003.

1. Chaturvedi N. K., Kumar S., Negi S., Tyagi R. K. (2010) Endocrine disruptors provoke differential modulatory responses on androgen receptor and pregnane and xenobiotic receptor: potential implications in metabolic disorders. Mol. Cell. Biochem. (ISSN: 0300-8177), 345(1-2): 291-308. (Impact factor: 2.329).

2. Kumar S., Jaiswal B., Kumar S., Negi S., Tyagi R. K. (2010) Cross-talk between androgen receptor and pregnane and xenobiotic receptor reveals existence of a novel modulatory action of anti-androgenic drugs. Biochem. Pharmacol. (ISSN: 0006-2952), 80(7): 964-976. (Impact factor: 4.748).

3. Negi S ., Akbarsha M. A., Tyagi R. K. (2008) Clinical correlates in drug-herbal interactions mediated via nuclear receptor PXR activation and cytochrome P450 induction. J. Endocrinol. Reprod. (ISSN: 0971 - 913X), 12(1): 1-12.

4. Negi S., Wahajuddin (2002) Pharmacy: The Authentic Profession, "Science Reporter", NISCAIR (CSIR), New Delhi, (ISSN: 0036-8512), 39 (1): 61-63.

5. Negi S ., et al. Transcriptional regulation of Pregnane & Xenobiotic Receptor by prospective anti-cancer herbal drugs. (Under submission)

Oral Presentation

1. ‘Anti-cancer herbal drugs as novel modulators of Pregnane and Xenobiotic Receptor (PXR): analysis via stable cellular models’ presented in National Conference on Recent Advances in Biosciences and Drug Discovery (RABDD-2014) during 03-04^th Mar., 2014 organized by Department of Pharmaceutical Sciences, Gurukul Kangri University, Haridwar, Uttarakhand, India. (Received the best oral presentation award)

2. ‘Modulation of xenobiotic sensing receptor PXR by prospective anti-cancer herbal drugs utilizing stable cellular model system’ presented in 6th International Symposium on Drug Metabolism and Pharmacokinetics (DMPK) during 27^th Feb. - 2^nd Mar., 2014 organized by NIPER, S.A.S Nagar, Mohali, Panjab, India.

3. ‘Anti-cancer herbal drugs as novel modulators of Pregnane and Xenobiotic Receptor (PXR): analysis via stable cellular models’ presented in18th Annual Convention of Society of Pharmacognosy (ISP-2014) on Promotion and Globalisation of Indian Herbal Products- Perspectives and Prospects during 21-22^nd Feb., 2014 organized by Department of Pharmaceutical Sciences, Maharshi Dayanand University, Rohtak, Haryana, India.

Poster Presentation

4. ‘Stable integration of PXR-promoter reporter DNA construct in human liver cell line: a tool to evaluate drugs, xenobiotics and endobiotics’ presented in 10^th Annual Research Festival (BIOSPARKS 2012) during 14-15^th Mar., 2012 organized by School of Life Sciences, Jawaharlal Nehru University, New Delhi.

5. ‘Stable integration of PXR-promoter reporter DNA construct in human liver cell line: a tool to evaluate drugs, xenobiotics and endobiotics’ presented inNational Conference & 30th Annual Symposium of the Society for Reproductive Biology and Comparative Endocrinology (SRBCE) on Novel Aspects and Emerging Trends in Reproduction and Endocrinology during 30^th Jan.-1^st Feb., 2012 organized by Dept. of Zoology, University College of Science, Mohanlal Sukhadia University, Udaipur, Rajasthan. (Received the best poster presentation award)

6. ‘New Chemical Entity (NCE) Hunt from Natural Resources: Antimicrobial Screening and Identification of Actinomycetes’ presented in International Conference on Organic Chemistry and Chemical Biology during 6-9^th Jan., 2006 organized at National Chemical Laboratory, Pune by Council of Scientific and Industrial Research (Govt. of India) and American Chemical Society, USA.

7. ‘Differential Expression and Induction of yejF gene in Salmonella typhimurium’ presented in National Conference on ‘ BIOTECH-2005’ during 22-24^th Dec., 2005 organized at Centre for Biotechnology, Jawaharlal Nehru University, New Delhi.

1. Participated in Flow cytometry workshop on ‘Bechman Coulter Flow Cytometer MoFlo Legacy’ during October 30-31, 2012 organized by AIRF-JNU, New Delhi and Beckman Coulter India.

2. Participated in the National workshop on ‘Computational Systems Biology and Dose Response Modeling’ during March 2-4, 2012 organized by Mahatma Gandhi-Doerenkamp Center for Alternatives to Use of Animals in Life Science Education, Bharathidasan University, Tiruchirappalli and The Hamner Institutes for Health Sciences, USA.

3. Attended National Workshop on Intellectual Property Rights during March 31-April 1, 2007 organized by Indian Institute of Technology, Kanpur in association with SIDBI Innovation and Incubation Centre.

4. Participated in International Symposium on Herbal Drug Research and Therapy during December 9th-10^th, 2006 organized at Vallabhbhai Patel Chest Institute, University of Delhi, Delhi.

5. Attended International Workshop under European Molecular Biology Organization (EMBO) World Programme on ‘ Cell Interactions in Development and Disease’ during December 16-18^th, 2004 organized at Centre for Cellular and Molecular Biology (CCMB), (CSIR) Hyderabad.

6. Attended the Workshop on ‘ Drug Discovery and Development in New Millennium: Interfacing with Pharmacological and Toxicological Experimentation’ during February 26-29, 2004 organized at National Institute of Pharmaceutical Education and Research (NIPER), Mohali (Punjab), India.

7. Attended WHO sponsored workshop on ‘Impact of TRIPS on pharmaceutical prices with specific focus on Generics in India’ at NIPER, Mohali.

8. Attended the Training Programme on ‘Intellectual Property Rights’ during June 23-24, 2003 organized by CSIR at RRL Srinagar (J&K).

9. Participated in ‘Sensitization Programme on Intellectual Property Rights’ during March 17^th, 2003 conducted at Noida by small Industries Service Institute, New Delhi in association with Patent Facilitating Centre, TIFAC, Department of Science & Technology, Govt. of India.

10. Attended the ‘53^rd Indian Pharmaceutical Congress - 2001’ during December 21-23, 2001 organized by Indian Pharmaceutical Congress Association in New Delhi.

11. Attended the National Seminar on ‘Recent Advances in Natural Products Chemistry’ during November 5-7, 2001 organized by Dept. of Chemistry, HNBG University, Srinagar (Uttaranchal).