The department has fully functioning 9 laboratories which is working 24 x7, with on call microbiologist.
A. Bacteriology Laboratory: Over last year this laboratory has processed 22963 Clinical Samples. The following test on routine basis
B. 1. Aerobic bacterial culture and sensitivity
2. Anaerobic bacterial culture and sensitivity
3. Gram’s Stain
4. Albert’s Stain
5. Automated blood culture and sensitivity
6. Automated bacterial identification and sensitivity system
7. Bacteriology of Water
1. The laboratory is also equipped with advanced automated system like Bact/Alert 3D Machine for automated Blood culture and culture of Mycobacterium from any sample. Along with it, it is also employing conventional methods for quality control and academic purpose. The laboratory has the necessary reference strains to carry out the tests in routine and for research purposes.
C. Serology Laboratory: This laboratory has processed 5056 Clinical sample in last year and does following test on routine basis of all required samples.
Besides the routine equipments the lab is equipped with automated system like Turbidimetry (ARC) machine, Nephalometer for accurate, quantitative and timely report.
D. Virology Laboratory: We have a fully equipped virology laboratory with continuous cell lines, inverted microscope and bio safety hood for academic activity .Besides that routine viral serology and fully automated ELISA reader and washer are performing following viral serology : Over last year this laboratory has processed 15394 Clinical Sample, and doing following test on routine basis of all required samples.
E. 1. HBV parameters :HbsAg ,HbeAg ,Anti HbS Antibody level
2. HBV viral load by qPCR
3. HCV serology
4. HCV viral load by qPCR
5. Dengue serology
6. Rubella Ig G ,IgM ELISA
7. Cytomegalovirus IgG and IgM ELISA
8. Herpes IgG and IgM ELISA
9. Tzank smear
D. Parasitology Laboratory:Over last year this laboratory has processed 2398 Clinical Sample, and doing following test on routine basis of all required samples.
1. Peripheral blood smear for malaria
2. QBC for Malaria
3. Rapid diagnostic test for Malaria
4. Routine Stool examination
5. Stool examination by concentration method
6. Modified ZN staining of Stool for coccidian parasite
7. Blood for microfilaria
8. Smear for Leishmania
9. Toxoplasma IgG and IgM ELISA
E. Immunology:Immunology laboratory has processed 614 Clinical in last year. Following test on routine basis of all required samples:
1. Anti CCPAnti TPO
2. Anti TTG
3. ANA by IFA
4. ANA profile
5. IgE quantitative level
The laboratory uses all the advanced technology for routine diagnosis and research projects and has standard machinery like Fluorescent Microscope, Dark ground Microscope, Phase contrast Microscope, Photographic Microscope with molecular backups.
F. Mycobacteriology Laboratory:The department of Microbiology, Subharti medical College has a state of art Mycobacteriology laboratory which is aNational Certified Diagnostic Laboratory for MDR TB diagnosis under RNTCP. It is also affiliated with DOTS centre. This laboratory has processed 1871 Clinical Sample, and doing following test on routine basis of all required samples:
1. ZN staining of all clinical sample for pulmonary and extra pulmonary tuberculosis
2. Fluorescent Staining of all clinical sample for pulmonary and extra pulmonary tuberculosis
3. Mycobacterial culture by automated method
4. Mycobacterial culture by conventional method
5. Mycobacterial Sensitivity for MDR/XDR detection by conventional method ,Automated and Line Probe Assay with all the molecular back up
6. TB PCR The laboratory follows the biosaftey norms and has the required machinery like Class II Bio Safety Hood, \ CO2 incubator and proper molecular back up.
G. Mycology Laboratory :Over last year this laboratory has processed 644 Clinical Sample, and doing following test on routine basis of all clinical samples
1. KOH mount
2. Indian Ink preparation for Cryptococcus
3. Fungal culture
4. Identification and sensitivity of all the clinically relevant fungal isolates.
It also has the necessary reference strains to carry out various tests in routine and for research purposes.
H. ICTC :The department of microbiology has a fully functional ICTC for HIV counseling and testing. The centre is recognized by NACO and is having a separate office with dedicated counselor, technician and all the facility required.
Over last one year ICTC has counseled and tested on 6729 subjects taking all the legal and ethical aspect of HIV testing into consideration.
H. HIC laboratory :The microbiology department looks after the infection control practice of Chatrapati Shivaji Sahuji Maharaja Hospital and takes care of supervision of routine waste segregation and disposal. Surveillance of quality indicator of the hospital under supervision of Principal, Subharti Medical College and Netaji Subhash Chandra Bose Medical College.
The hospital infection control committee takes classes and training activities of all the nursing and paramedical staff to ensure that knowledge of the staff is upto date regarding infection control practice. Besides the infection control, the team is carrying out all the routine surveillance and microbiological quality testing of all the operation theatres ,intensive care units and all the required staffs. HICC has also prepared the antibiotic policy for reference by all physician of Chatrapati Shivaji Sahuji Maharaja Hospital.
Molecular Lab:
J. The Molecular Biology Laboratory at Department of microbiology, Subharti Medical College, Meerut offers services for Molecular testing of infectious diseases with facilities of nucleic acid isolation and amplification techniques like PCR/ gel electrophoresis etc.
Our laboratory is 24 x 7 functional with well equipped Biosafety level 3 facilities for detection of Bacterial , Fungal, Viral, Parasitic infectious agents.
Our Molecular Microbiology Laboratory services are accredited by the National Accreditation Board for Testing and Calibration Laboratories (NABL) (ISO 15189:2012) since May 2020. Our lab is among one of the few labs which are NABL accredited lab for both Real- Time RT- PCR (open system) and True Nat (close system).
1. HEPATITIS B VIRAL LOAD
2. HEPATITIS C VIRAL LOAD
3. DENGUE/ CHICKUNGUNYA PCR
4. HPV- PCR
5. SALMONELLA PCR
6. COVID-19 PCR
List of Instrument present in Department of Microbiology :
(With Unit wise distribution)
Bacteriology &Media :
S.N.
Equipment
Department
Clinical Lab
1.
Binocular Microscope
-
02
2.
Fridge
-
04
3.
Incubator
01
03
4.
Candle Jar
02
01
5.
Vitek – 2 Compact
-
01
6.
Automated Blood culture System: Bact /Alert – 3D
01(TB Lab)
01
7.
Deep freezer
-
01
8.
Biosafety cabinet
-
01
9.
Micrometry Unit –Micrometer Eye piece & Stage
01
01
10.
ATCC Strains E. coli 25922
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√
11.
ATCC Strains Stahylococcus aureus 25923
√
√
12.
ATCC Strains Pseudomonas aeruginosa 27853
√
√
13.
ATCC Strains Klebsiella pnuemoniae 700603 (ESBL)
√
√
14.
ATCC Strains Staphylococcus aureus 43300(MRSA)
√
√
15.
ATCC Strains H. influenzae 19418
√
√
16.
ATCC Strains Enterococcus faecalis 29212
√
√
17.
ATCC Strains Staphylococcus aureus 29213 (Vancomycin MIC )
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√
18.
Anti sera Shigella dysenteriae (Polyvalent A) DENKA SEIKEN Co.LTD
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√
19.
Anti sera Shigella dysenteriae (Polyvalent A1) DENKA SEIKEN Co.LTD
√
√
20.
Anti sera Shigella flexeneri (Polyvalent B) DENKA SEIKEN Co.LTD
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√
21.
Anti sera Shigella boydii (Polyvalent C) DENKA SEIKEN Co.LTD
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√
22.
Anti sera Shigella boydii (Polyvalent C1) DENKA SEIKEN Co.LTD
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√
23.
Anti sera Shigella boydii (Polyvalent C2) DENKA SEIKEN Co.LTD
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√
24.
Anti sera Shigella boydii (Polyvalent C3) DENKA SEIKEN Co.LTD
